|
||||||||||||
The ultimate thermodynamic
modelling spreadsheet for Excel
|
||||||||||||
These are references in which MeltCalc has been described, or used for assay optimization or that show additional considerations concerning thermodynamic probe design.
BioTechniques
1999;27:1218 - Inital description of program and application to PCR Clin Chem 1999;45:2094 - Application to APO-B and considerations of specificity of hyb.-probes Clin Chem 2000;46:1728 - Application to TPMT, three color multiplexing, limitations of hyb-probes Clin Chem 2000;46:1939 - Thermodynamics of bulges (insertion/deletion) examplified with UGT1A1 polyporphism Clin BioChem 2000;33:535 - Thermodynamical optimization of color multiplexing (FV-Leiden and MTHFR) Clin Chem 2001;47:1331 - Published pitfalls in genotyping: Thermodynamic considerations Clin Chem 2001;47:1956 - Effect of Mg, DMSO, SybrGreen on Tm in PCR, modelling of that and other Tm formulas Clin Chem 2004;50:2182 - Color multiplexing for 1-tube Genotyping and Splice Variant detection Clin Chem 2006;52:1426 - Color and Temperature multiplexing for complex 1-tube PrP Genotyping J Mol Diag 2007;9:345 - Improved real-time PCR using NN-Calculations Clin Chem 2008;54:990 - Design of non-continous binding probes for haplotyping Anal Biochem 2009;385:143 - Thermodynamics of High-Resolution Melting |
||||||||||||