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The ultimate thermodynamic modelling spreadsheet for Excel™

MeltCalc is copyright by:
Ekkehard Schütz and Nicolas von Ahsen.
All rights reserved.
These are references in which MeltCalc has been described, or used for assay optimization or that show additional considerations concerning thermodynamic probe design.

BioTechniques 1999;27:1218 - Inital description of program and application to PCR
Clin Chem 1999;45:2094 - Application to APO-B and considerations of specificity of hyb.-probes
Clin Chem 2000;46:1728 - Application to TPMT, three color multiplexing, limitations of hyb-probes
Clin Chem 2000;46:1939 - Thermodynamics of bulges (insertion/deletion) examplified with UGT1A1 polyporphism
Clin BioChem 2000;33:535 - Thermodynamical optimization of color multiplexing (FV-Leiden and MTHFR)
Clin Chem 2001;47:1331 - Published pitfalls in genotyping: Thermodynamic considerations
Clin Chem 2001;47:1956 - Effect of Mg, DMSO, SybrGreen on Tm in PCR, modelling of that and other Tm formulas
Clin Chem 2004;50:2182 - Color multiplexing for 1-tube Genotyping and Splice Variant detection
Clin Chem 2006;52:1426 - Color and Temperature multiplexing for complex 1-tube PrP Genotyping
J Mol Diag 2007;9:345 - Improved real-time PCR using NN-Calculations
Clin Chem 2008;54:990 - Design of non-continous binding probes for haplotyping
Anal Biochem 2009;385:143 - Thermodynamics of High-Resolution Melting